Trichoderma viride in bipolaris oryzae management journal of biopesticides 31 special issue 093 095 2010 93 the biological control of paddy disease brown spot bipolaris oryzae by using trichoderma viride in vitro condition s. Bipolaris oryzae cpc 28826 oryza sativa sanpatong, chiang mai mf490809 mf490831 mf490853 cpc 28828 oryza sativa mae hia agricultural research, demonstrative and training center, chiang mai mf490810 mf490832 mf490854 bipolaris setariae cpc 28802 imperata cylindrica chiang mai university, chiang mai mf490811 mf490833. Phylogenetic analysis based on its sequences and conditions. The major diseases are blast pyricularia oryzae, bacterial blight, sheath rot sarocladium. Ten 10 irrigated and upland rice varieties were used in this study. Hence a detailed systematic investigation was conducted to test in invitro antifungal activity against important seed borne pathogens bipolaris oryzae of paddy.
Race t no longer considered a threat since the transition to normal cytoplasm corn 2. Evaluation of rice genotypes for resistance against brown. Ahn ip, kim s, kang s, suh sc, lee yh 2005 rice defense mechanisms against cochliobolus miyabeanus and magnaporthe grisea are distinct. In order to reveal the photomorphogenic response and to identify new genes upregulated by nuv irradiation, suppression subtractive hybridization ssh was carried out in b. Pdf brown leaf spot of rice, caused by bipolaris oryzae, is one of the most destructive. Infected seeds usually provide the primary inoculum. The inheritance of light dependence for conidial development inbipolaris oryzae was analyzed using singleascospore isolates. Identification and expression analysis of regulatory genes. Seed borne and transmission of bipolaris oryzae, the. When a photoinduced strain was crossed with another photoinduced strain, only photoinduced progeny were produced. Results of this study showed that compound 1 and 2 exhibited a very strong inhibitory activity against tested fungi with minimum inhibitory. We isolated and characterized t4hr1 gene encoding 1,3,6,8tetrahydroxynaphthalene 1,3,6,8thn reductase, which converted 1,3,6,8thn to scytalone in the melanin biosynthesis from b. This disease was the causal agent of the bengal famine of 1943. Inheritance of photocontrol of conidial development in the.
Foliar application of fungicides for the management of. In a favorable environment, conidia are produced in brown portions of the lesions and figure 1 switchgrass leaf with bipolaris infection. Seed borne and transmission of bipolaris oryzae, the causal. Bipolaris oryzae is the causal agent of brown spot disease in rice and produces the dark pigment melanin. Mycelium are germinate from two end of the conidia.
Most bipolaris species are associated with leaf spot or blight, root rot, ear rot, seedling blight, and other diseases of cultivated and wild gramineous plants 6,7. Pcr by adding double sterilized nuclease free water. Pathogens free fulltext etiology and symptoms of maize. Bipolaris oryzae oryza sativa, mr 219 malaysia madhya pradesh bomp1 2010 bipolaris oryzae oryza sativa, p 14 60 madhya pradesh bomp2 2010 bipolaris oryzae oryza sativa, pusa kranti madhya pradesh bomp3 2010 bipolaris oryzae oryza sativa, swarna west bengal bowb1 2008 bipolaris oryzae oryza sativa, kankaj west bengal bowb2 2008. In order to reveal the photomorphogenic response and to identify new genes upregulated by nuv irradiation, suppression subtractive hybridization ssh was carried out. Seed borne and transmission of bipolaris oryzae, the causal pathogen of brown spot of rice vu van ba1 and somsiri sangchote2 abstract rice brown spot bipolaris oryzae, in paddy fields was investigated to find the relationship between disease severity on flag leaf and kernel infection, at three growth stages of flowering, milky, and dough stages. This disease was the causal agent of the bengal famine of 1943 it was considered for use by the usa as a biological weapon against japan during world war ii. When a photoinduced strain was crossed with a nonphotoinduced i strain, photoinduced and nonphotoinduced i. There are three races of this pathogen race o, race t, and race c.
A phylogenetic and taxonomic reevaluation of the bipolaris. When a photoinduced strain was crossed with a nonphotoinduced i strain, photoinduced and nonphotoinduced i progeny were produced in a ratio of 1. Its conidiophore is brown in color and remain as straight or slightly curved. Photosynthesis and sugar concentration are impaired by the. This disease has great economic importance because bengal famine was occurred in 1942 and 2 million people died in that area due to yield loss of 60 90%. Abstract bipolaris oryzae and gerlachia oryzae, which cause rice brown spot and leaf scald, respectively, are mainly disseminated by seeds. A phylogenetic and taxonomic reevaluation of the bipolaris cochliobolus curvularia complex dimuthu s. Seed samples were collected from the rice experimental field comprised of different rice genotypes at national rice research. A system for inducing sporulation of bipolaris oryzae. Melanin is a darkpigmented polymer that protects organisms against environmental stress, and its production is also widespread in the fungal kingdom. Bipolaris oryzae is the causal agent of brown leaf spot disease in rice, and its asexual spore conidium formation is known to be induced by nearultraviolet nuv irradiation. Bipolaris oryzae is the causal agent of rice brown spot disease and is.
This fungus can infect both mature rice plant and seedlings of rice. In vitro evaluation of fungicides against bipolaris. Insertional mutagenesis and characterization of a polyketide. Biocontrol and seed transmission of bipolaris oryzae and. Diversity of the rice brown spot pathogen, bipolaris oryzae, in. International journal on advanced science, engineering and. The infection was acquired after diesel oil splashed into the patients right eye while he was working in a paddy field. It was considered for use by the usa as a biological weapon against japan during world war ii. Different types of metal nanoparticles and nanoformulations have been. Bipolaris oryzae strain d9f669 a stock culture at the laboratory of plant pathology, shimane university was used as the wildtype strain. Pelagia research library plant extracts are screened to detect secondary metabolites with biological activities, including antifungal activity.
In this paper, we report 46 newly identified genes that were upregulated by nuv irradiation in b. The genus bipolaris includes a number of significant plant pathogens with worldwide distribution. New species and records of bipolaris and curvularia from thailand. Bipolaris oryzae causes brown spot in rice oryza sativa inflicting substantial grain yield losses worldwide. Bipolaris oryzae, the causal agent of brown spot disease, is an economically important pathogen of rice found in several rice growing countries in the world. Bipolaris oryzae can survive as mycelium in soil for many years without a host and is also seedborne. Bipolaris anamorph of the ascomycetous genus cochliobolus, which has more than 100 species, is an important genus of plant pathogens 4,5,6,7,8. Genetic diversity and population structure among isolates. Knowledge of the population structure, genetic diversity and sexual recombination of the. In order to control this disease, three phenolic antioxidants were tested. Brown spot, caused by bipolaris oryzae, is an economically important disease in rice because it negatively impairs grain quality and reduces yield by up to 74% kohls et al. In order to control this disease, three phenolic antioxidants were tested salicylic acid, benzoic acid and hydroquinone.
The antifungal activity of the tested substances were investigated against b. Bipolaris maydis, also known as drechslera maydis or cochliobolus heterostrophus, causes southern corn leaf blight sclb and stalk rot 1. Pathogen collected from infected rice leaves were thoroughly washed with tap water. The name helminthosporium oryzae was changed to bipolaris oryzae because of the bipolar conidial germination shoemaker, 1959. Antifungal activity of biphenyls from streptomyces sp.
Conidia are usually curved and tapering towards the end. These species are commonly associated with leaf spots, leaf blights, melting outs, root rots, foot rots and other disease symptoms mainly in high value field crops in the family poaceae, including rice, maize, wheat and sorghum and on various other host plants. Bipolaris setariae gu073108 bipolaris portulacae ay004779 bipolaris zea af081452 bipolaris indica af081449 bipolaris sorghicola jq517488 bipolaris sorokiniana jn128888 bipolaris specifera jq585669 jq585694 bipolaris oryzae jq585685 jq965127 jq585692 bipolaris australiensis jq585665 jq965129 jq585695. Bipolaris oryzae used in this study were isolated from rice field soil and infected rice plants respectively. Fungal strains and growth conditions bipolaris oryzae strain d9f669 stock culture at the laboratory of plant pathology, shimane university was used as the wt strain. These samples from 25 districts were used for isolation of desired pathogen from spotted leaf and seeds with infection of brown spot disease. Brown spot caused by bipolaris oryzae is an important rice disease which can cause a reduction in yield and grain quality in malaysia. Benzoic acid occurs naturally free and bound in many plant and animal species. A collection of 348 bipolaris isolates was made from cultivated rice paddies and weeds in mazandaran, guilan, golestan, khuzistan and fars provinces, during 2011 and 2012.
Brown spot of rice is caused by the pathogen named bipolaris oryzae imperfect stage or cochliobolusbolus miyabenus perfect. Brown spot disease is an important disease of rice in the world and iran. Bipolaris oryzae, a novel fungal opportunist causing. Pdf morphomolecular diversity of bipolaris oryzae causing brown. Bengal area, which resulted in two million people died because of the famine.
Nomenclature of drechslera and bipolaris, grass parasites segregated from helminosporium. Bipolaris oryzae is known as a significant causal agent of brown spot disease in rice pitt et al. Bipolaris oryzae, oryza sativa, plant extract, antimicrobial activity, medicinal plants. Expression of t4hr1, a 1,3,6,8tetrahydroxynaphthalene. Xiao jz, tsuda m, doke n, nishimura s 1991 phytotoxins produced by germinating spores of bipolaris oryzae. Antifungal potentiality of some medicinal plant extracts. Research article 294 assessment of seed infestation level of. Pdf isolation, screening and identification of virulent. Bipolaris sorokiniana an overview sciencedirect topics.
Bipolaris oryzae is the causal agent of rice brown spot disease and is responsible for significant economic losses. Pdf behaviour of bipolaris oryzae at different temperatures. Population structure, genetic diversity, and sexual state. Pdf pathogenic variability of bipolaris oryzae causing leaf. Bipolaris oryzae, rice brown spot, pathogenicity, agarosesynergel. Many species of the genus bipolaris are important plant pathogens and often cause leaf spot, root rot, and seedling blight in an extremely wide range of hosts around the world. Two opsinlike genes, ops1 and ops2, were identified from a subtracted cdna library for the identification of nearuv nuv radiationenhanced genes using suppression subtractive hybridization methods in the brown leaf spot fungus bipolaris oryzae. This disease was the causal agent of the bengal famine in 1943 2. Pdf brown spot of paddy caused by bipolaris oryzae is a threat to paddy cultivation.
Cloning and expression analysis of two opsinlike genes in. New species and records of bipolaris and curvularia from. These samples from 25 districts were used for isolation of desired pathogen from spotted leaf and seeds with infection of. Sporulation of isolates of bipolaris oryzae on rabbit food agar with 12 hr of near uv light followed by 12 hr of complete darkness isolate sporulation on rfaa lr2172 9. Helminthosporium oryzae destroyed 50 to 90% of the rice crops in the 1 pest in the ippc contexts refers to any species, strain or biotype of plant, animal or pathogenic agent injurious to plants or plant products. Control of brown spot pathogen of rice bipolaris oryzae using.
Control of brown spot pathogen of rice bipolaris oryzae. Plants contain hundreds or thousands of metabalites. Cochliobolus miyabeanus cochmioverview eppo global database. Potatodextrose agar pda plates were inoculated with a small mycelial plug at the center, followed by incubation at 25 1 c in darkness. In recent years, maize leaf spot caused by bipolaris species has frequently occurred with complex symptoms and is becoming increasingly serious in sichuan province of china. One taxonomic characteristic of bipolaris species is the bipolar germination of conidia, but conidia of bipolaris oryzae, the causal pathogen of. In general, melanin accumulates in fungal cell walls and has been believed to confer tolerance to environmental stresses such as uv radiation. The ops1 and ops2 genes encode predicted proteins of 306 and 304 amino acids, respectively. Research article 294 assessment of seed infestation. Regional survey and identification of bipolaris spp. Pests and diseases cause annual yield loss upto 40 % in rice crop srinivasachary et al. Bipolaris oryzae, fusarium semitectum, fusarium fujikuroi and curvularia lunata was analyzed. Pdf pathogenic variability of bipolaris oryzae causing. Brown spot caused by bipolaris oryzae is one of the destructive diseases of rice in the world and causes severe losses in grain quality and quantity.
For the combined dataset all free modal parameters were obtained. Bipolaris maydis c5 14112 af0725 af0880 berbee et al. The application of chemical agents in controlling the brown spot disease can cause undesirable effects such as residual toxicity, environmental pollution, and development of pathogen resistance to fungicides. Cochliobolus miyabeanus formerly known as helminthosporium oryzae is a fungus that causes brown spot disease in rice. An assessment of rice crop resistance to bipolaris oryzae, a causal agent of rice brown leaf spot or helminthosporium disease was carried out. Morphological and phylogenetic investigation of bipolaris. Bipolaris is a genus of fungi belonging to the family pleosporaceae. The fungus was described as drechslera oryzae based on the amphigenous conidial germination subaramanian and jain, 1966.